# Phase Retrieval Phase Retrieval is the process of extracting the phase shift, induced by the sample on the beam, acquired on the detector. This phase shift is then used to reconstruct a map of the (deviation from unity of the real part of) refractive index through tomographic reconstruction. Nabu implements the following non-iterative phase retrieval methods. ## Paganin phase retrieval This phase retrieval method was first described in [[1]](#references). The implementation [PaganinPhaseRetrieval](apidoc/nabu.preproc.phase) follows the normalization of [[2]](#references). The nabu implementation of Paganin phase retrieval should be compatible with the one of [tomopy](https://github.com/tomopy/tomopy). More precisely, given a `delta_beta` value, the result should be the same as the one provided by tomopy with ```python extracted_phase_tomopy = retrieve_phase( data, pixel_size=pixel_size_meters*1e2, dist=distance_m * 1e2, energy=energy_kev, alpha=1./(4*3.141592**2 * delta_beta), ) ``` there is notably a factor of `1/(4*pi**2)` between the conventions. ```{warning} Since version 2021.1.0, lengths in PaganinPhaseRetrieval are expressed in meters (distance and pixel size). ``` ## Single-Distance CTF phase retrieval This method, although based on Contrast Transfer Function (CTF) rather than Transport of Intensity (TIE), can also be expressed as a single step "filtering" processing. See for example [[2]](#references). This method is implemented in [CTFPhaseRetrieval](apidoc/nabu.preproc.ctf). ```{note} Lengths are expressed in meters ``` To build a `CTFPhaseRetrieval`, one first needs to describe the setup geometry. This is done with the [GeoPars](apidoc/nabu.preproc.ctf) class: ```python geo_pars = GeoPars( z1_vh=None, z2=z2, pix_size_det=pix_size_det, length_scale=length_scale, wavelength=wavelength, ) ``` once this `GeoPars` object is built, the `CTFPhaseRetrieval` object can be created and called: ```python ctf_filter = CTFPhaseRetrieval( geo_pars, delta_beta, lim1=1.0e-5, lim2=0.2, ) phase_image = ctf_filter.retrieve_phase(projection, normalize_by_mean=True) ``` ## Unsharp masking Single-distance phase retrieval tend to blur the images. To recover sharp features, a common method is to use a [unsharp mask](https://en.wikipedia.org/wiki/Unsharp_masking) image processing step. This feature is implemented in [nabu.misc.unsharp](apidoc/nabu.misc.unsharp), and in the Cuda/Opencl back-ends [nabu.preproc.unsharp_cuda](apidoc/nabu.misc.unsharp_cuda) and [nabu.preproc.unsharp_opencl](apidoc/nabu.misc.unsharp_opencl) respectively. ## Enable phase retrieval from the configuration file To enable the phase retrieval processing step, modify the section `[phase]` of [nabu config file](nabu_config_file.md): ```ini [phase] method = Paganin # possible values: none, Paganin, CTF ``` additional parameters can be specified: ```ini # delta/beta ratio for the Paganin method paganin_delta_beta = 100.0 # Padding type for the filtering step. Available are: mirror, edges, zeros paganin_padding_type = mirror ``` To enable [unsharp masking](#unsharp-masking), the value of `unsharp_coeff` must be greater than zero. ```ini # Unsharp mask strength. The unsharped image is equal to # UnsharpedImage = (1 + coeff)*originalPaganinImage - coeff * ConvolutedImage. Setting this coefficient to zero means that no unsharp mask will be applied. unsharp_coeff = 0 # Standard deviation of the Gaussian filter when applying an unsharp mask # after the Paganin filtering. Disabled if set to 0. unsharp_sigma = 0 ``` ## References [1] D. Paganin Et Al, "Simultaneous phase and amplitude extraction from a single defocused image of a homogeneous object", Journal of Microscopy, Vol 206, Part 1, 2002 [2] Boliang Yu Et Al, "Evaluation of phase retrieval approaches in magnified X-ray phase nano computerized tomography applied to bone tissue," Opt. Express 26, 11110-11124 (2018)